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The LS&E Flow Cytometry unit the invites You to attend special seminar on “Imaging Flow Cytometry (IFC) using the Image Stream“. Date: Wednesday, July 7th, 10:00 o’clock, Location: Biology faculty auditorium, Technion Speaker: Dr. Yael Lupu-Haber, LSE application specialist for the Image Stream Title: Imaging Flow Cytometry (IFC) using the Image Stream Abstract: IFC combines speed, sample size and phenotyping abilities of flow cytometry with the morphological analysis ability of microscopy in a single instrument platform. It allows statistical analysis of cellular morphology, fluorescent intensity and texture. IFC applications include: Cell signaling, Internalization ,Co-localization ,Microbiology ,Cell cycle ,Apoptosis ,Shape change ,Cell-cell interactions ,Oceanography. Looking forward to seeing you all, LSE Flow Cytometry team...
June 28th, 13:00 – Special seminar on IN Cell Analyzer – New platform to meet your High Content Analysis needs
The LS&E Microscopy core facility the invites You to attend special seminar on “IN Cell Analyzer – New platform to meet your High Content Analysis needs“. Date: Wednesday, June 28th, 13:00 o’clock, (45 min’s talk) Location: Biology faculty auditorium, Technion Speaker: Dr. Przemysaw Fleszar, Application Specialist, Cell Analysis, GE Healthcare Title: IN Cell Analyzer – New platform to meet your High Content Analysis needs Abstract: Recent developments in the IN Cell portfolio enable powerful high content analysis (HCA) capabilities on live cell assays, using widefield deconvolution, line scan confocal and transmitted light imaging modalities. These cutting edge hardware platforms use class leading technologies in concert with sensitive and powerful image analysis platforms to give the user the statistical power to address biological questions in cell biology that previously lay out of reach. Results on the microscopic scales ranging from tissues, cell populations down to single cells, investigating molecular organization of individual cells in even the most sensitive of live samples. We will present GE Healthcare’s latest advances in high content imaging technologies, integrated into the new IN Cell Analyzer platforms and our new easy-to-use IN Carta analysis software package. During the session, we will present how to automate image acquisition processes and how to quickly and confidently extract multi-parameter data from thes...
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies. The next seminars will be on “Clearing methodologies for biological samples“. Date: Tuesday, April 25th, 10:30 o’clock, (45 min’s talk) Location: Room 4-17, Emersson Building Speaker: Shlomi Lazar, Ph.D. Division of medical chemistry, The Israel institute for Biological Research (IIBR). Title: Looking inside the organs: a comparative analysis of three methods for establishing optically transparent samples Abstract: Typical histological study, using sectioning of the tissue, has major limitations in obtaining 3D images of structural components and cells distribution within tissues. Ideally, samples should be imaged at high spatial resolution with minimal sectioning. However, thick tissue imaging is limited mostly because of light scattering. In this study, we compared the efficacy of three recently published clearing protocols (Scale, 3DISCO and Clarity) in generating a transparent thick section which can be subjected to confocal analysis. Brains, ovaries and embryos obtained from transgenic mice expressing enhanced yellow fluorescent protein (eYFP) in specific cells or wild type mice, were collected and cut to thick sections (2-3mm). Some slices were first fluorescently immunolabeled for various markers. All sections were processed in one or more of the following protocols: (a) The Scale protocol ...
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies. The next seminar will be on “Insights into super-resolution – methodologies and applications of super-resolution microscopy “. Date: Tuesday, March 21st, 10:00 o’clock Please keep note that the seminar will be 30 minutes earlier than the original plan. Location: Auditorium , Faculty of Biology Speaker: Avi Jacob, Ph.D. Head of Light Microscopy facility, Bar-Ilan University. Abstract: Microscopy-based methods for breaking diffraction limited resolution are collectively known as super-resolution (SR). There are several methods which can be divided into two main approaches: deterministic and stochastic. Stochastic methods include all localization techniques such as STORM and deterministic methods include the optical approaches such as STED. STED (STimulated Emission Depletion) is a mature technology, yet can require optimization to reach ~50nm resolution. Recently, companies have begun to offer “hardware-optimization + software based SR”, whereby a measure of SR is achieved by automatically optimizing acquisition and then submitting to deconvolution. Each has their advantages. Model systems need to be appropriate for SR, such as sub organelle structures, nuclear pores or fine membrane studies. Also, care must be taken to ensure that SR is technically possible taking into account the specific SR method ...