OUR SERVICES & UNITS
NEWS & EVENTS
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies. The next seminars will be on “Clearing methodologies for biological samples“. Date: Tuesday, April 25th, 10:30 o’clock, (45 min’s talk) Location: Room 4-17, Emersson Building Speaker: Shlomi Lazar, Ph.D. Division of medical chemistry, The Israel institute for Biological Research (IIBR). Title: Looking inside the organs: a comparative analysis of three methods for establishing optically transparent samples Abstract: Typical histological study, using sectioning of the tissue, has major limitations in obtaining 3D images of structural components and cells distribution within tissues. Ideally, samples should be imaged at high spatial resolution with minimal sectioning. However, thick tissue imaging is limited mostly because of light scattering. In this study, we compared the efficacy of three recently published clearing protocols (Scale, 3DISCO and Clarity) in generating a transparent thick section which can be subjected to confocal analysis. Brains, ovaries and embryos obtained from transgenic mice expressing enhanced yellow fluorescent protein (eYFP) in specific cells or wild type mice, were collected and cut to thick sections (2-3mm). Some slices were first fluorescently immunolabeled for various markers. All sections were processed in one or more of the following protocols: (a) The Scale protocol ...
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies. The next seminar will be on “Insights into super-resolution – methodologies and applications of super-resolution microscopy “. Date: Tuesday, March 21st, 10:00 o’clock Please keep note that the seminar will be 30 minutes earlier than the original plan. Location: Auditorium , Faculty of Biology Speaker: Avi Jacob, Ph.D. Head of Light Microscopy facility, Bar-Ilan University. Abstract: Microscopy-based methods for breaking diffraction limited resolution are collectively known as super-resolution (SR). There are several methods which can be divided into two main approaches: deterministic and stochastic. Stochastic methods include all localization techniques such as STORM and deterministic methods include the optical approaches such as STED. STED (STimulated Emission Depletion) is a mature technology, yet can require optimization to reach ~50nm resolution. Recently, companies have begun to offer “hardware-optimization + software based SR”, whereby a measure of SR is achieved by automatically optimizing acquisition and then submitting to deconvolution. Each has their advantages. Model systems need to be appropriate for SR, such as sub organelle structures, nuclear pores or fine membrane studies. Also, care must be taken to ensure that SR is technically possible taking into account the specific SR method ...
The LS&E Microscopy core is delighted to announce on seminars series on advanced microscopy applications and methodologies for Life Sciences Imaging taking place the biology faculty over the coming months. These seminars are designed as a program of short talks to enrich your knowledge on Live cell imaging, Clearing methodologies , Super-resolution and Image analysis aimed to help you optimize your microscopy imaging workflow. These seminars are free of charge, however, registration in event form is required. Looking forward to seeing you all, LS&E microscopy core team. ...
The LS&E microscopy unit is happy to announce that HIVE technology is available for IMARIS and Light-sheet users. HIVE is a high speed centralized data repository that removes the need to constantly move or duplicate data sets in processing and analysis workflows The HIVE is a modular platform (computation & storage server) optimized for image processing and data handling. The HIVE designed for the pragmatic, biology-focused scientist, it boosts productivity in the LS&E microscopy unit .Its modular design integrates high speed processing, visualization, remote access, flexibility, data security, scalability and ease of use in one unit....