Description

BD FACSAria-IIIu cell sorter is a new digital sorter enabling high-speed sorting and multicolor analysis.

The instrument accommodates 4 air-cooled lasers at 488-nm, 633-nm, 407-nm and a new laser – 561 nm

Sorting at up to 15,000 events/second

Multicolor analysis of up to 15 parameters

Up to four-way bulk sorting devices for a variety of tube sizes.

Automated Cell Deposition Unit (ACDU) for sorting to multi-well plates or microscope slides

 

Features

Optical System: Patented BD OctagonTM and TrigonTM technology.

Laser excitation and filters (see FACSAria III configuration in the related links):
Lasers: 488-nm, 633-nm, 561nm, and 407-nm.
15 parameters: forward scatter, side scatter, 5 fluorescent detectors of 488-nm, 2 fluorescent detectors of 633-nm, 4 fluorescent detectors of 561-nm, and 2 fluorescent detectors of 407-nm.

 

Laser line/color (Excitation)	Detector	Emission Filter (nm)	Example of available markers
407 nm  Violet	A	695 /40 (Far red)	QD-710
	B	450/40 (Blue)	DAPI, Hoechst 33258, Pacific Blue, BV421, Alexa Fluor 405
488 nm  Blue	A	780 / 60 (Infra-Red)	PE-Cy7
	B	695 / 40 (Red-FarRed)	PE-Cy5, PerCP, PerCP-Cy5.5, 7-AAD, PI
	C	616/23 (Red)	PE-Texas Red, PI
	D	585 / 42 (Orange-Red)	PE, PI
	E	530 / 30 (Green)	Alexa Fluor 488, FITC, GFP, CFSE, Sytox Green, YFP
	F	488 / 10	SSC
			FSC
561 nm  Yellow Green	A	780 / 60 (Infra-Red)	PE-Cy7
	B	670/14 (Far Red)	7-AAD , PE-Cy5
	C	610/20 (Red)	mCherry, PE-CF594 , PI
	D	582/15 (Orange-Red)	Cy3, PE
633nm  Red	A	780/60 (Infra-Red)	APC-Cy7
	B	660/20 (Red)	APC

 

 

Additional available filters:

 

Mirror	450LP	5005LP	530LP	635LP	685LP	735LP
Band pass	405/20	525/50	530/30	585/42	610/20	670/14	675/20	695/40

 

Specifications and performance

 

Fluorescence Sensitivity

FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)

PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

 

Fluorescence Resolution

Coefficient of variation PI-Area of <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei

Coefficient of variation Hoechst-Area of <3.5%, full G0/G1 peak for Hoechst-stained chicken erythrocyte nuclei

 

Fluorescence Linearity

Doublet/singlets ratio for CEN stained with PI = 1.95-2.05 detected off the 488-nm laser or Hoechst = 1.95-2.05 detected off the 407-nm laser

 

Forward and Side Scatter Sensitivity

Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and 0.5-micron beads.

 

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

 

Sample Acquisition Rate

Maximum acquisition rate: 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate < 70,000 events per second with more parameters, > 100,000 events per second with fewer parameters.

 

Sorting options:

Two- and four-way sorting into microtubes, 12 X 75, and 15 mL tubes

ACDU (automated cell deposition unit) for sorting into multi-well plates and onto microscope slides

Sample injection chamber for various sample input tubes, including microtubes, 12 X 75, and 15 mL tubes. Includes sample agitation and temperature control.

 

Signal Processing

• Digital electronics with 10 MHz sampling frequency
• Up to sixteen acquisition channels with up to four spatially separated laser beams
• 18-bit data acquisition for area signals
• Inter- and intra-beam compensation on-line and post-acquisition off-line
• Height, width, and area measurement available for all parameters
• Time parameter available for kinetic studies (Calcium)
• Ratio measurements between any two parameters from any laser

 

Nozzle selection and design

An expanded set of nozzles lets users sort a wide range of particle sizes. Four nozzle sizes are supported: 70, 85, 100, and 130 microns.

 

Working policy

Users who wish to work on the FACS-Aria-IIIu must contact Dr. Aviv Lutaty before ordering this system.

 

 

Related Documents

AriaIII configuration