Author: Nitsan Dahan

22 June – the newest ZEISS Light-Sheet 7 would be installed at the LS&E this year.

The LS&E Microscopy Core Facility Center is pleased to announce that the newest ZEISS Light-Sheet 7 would be installed at the LS&E this year.   The new Light-Sheet 7 microscope is dedicated for multi-view imaging for Living sample and as well as for Optically Cleared Specimens. The exceptional stability of Light-Sheet 7 enables to observe living samples over extended periods of time – even days – with minimal photo-toxicity than ever before. In addition, the new light sheet microscope can image very large optically cleared specimens with subcellular resolution. Light-Sheet 7 designed to image specimens at up to 2 cm in size at any refractive index between 1.33 and 1.58, and in almost all clearing solutions. The Light-Sheet 7 have dedicated optics, sample chambers and sample holders to match accurately the desired refractive index for multiplicity clearing method with the ability to image large samples or cleared organ/tissue such as mouse brain,...

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LS&E Day for Image Processing & Analysis

On Dec. 25th @ Room 300 Biotechnology & Food Eng., Technion Agenda: 09:00-09:15 Gathering & coffee 09:15-09:30 LS&E infrastructure overview 09:30-11:00 Introduction to image processing & analysis 11:00-11:20 Coffee break 11:20-12:00 Advanced de-convolution (Dr. Douglas Lutz) 12:00-12:50 Image analysis tools (Fiji, Ilastik, Imaris, CellProfiler) & applications 12:50-13:00 Summary & New @ The LSE Registration is needed. Please register here LSE DAY for IA . For more information, please contact Dr. Yael Lupu Haber: yaelupu@technion.ac.il , 1386  ...

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24 Feb – Arivis Workshop : Seminar, Live Demo and Hands On session

Dear all, The LS&E microscopy and image analysis core facility is happy to invite you to participate on Arivis Workshop. A workshop on Large image data, visualization, image processing and analysis. General agenda, o Sunday, Feb 24, 11:00 - in Emerson building floor 6 at Aquarium Room, general presentation and Live demo, open to everyone o Feb 24 afternoon: Hands-on session of arivis InViewR and arivis Vision4D, by registration only - ndahan@technion.ac.il Please find out more details on the enclosed PDF. For more information, Please contact: Dr. Nitsan Dahan, 077-8871385, ndahan@technion.ac.il Dr. Yael Lupu-Haber, 077-8871386, yaelupu@technion.ac.il   Looking forwards to see You, The Microscopy Team...

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Single Cell workshop at the Technion Genome Center

Dear all, We are happy to invite you to a Single Cell Sequencing Applications Workshop at the Technion Genome Center. The workshop will take place on February 27th, at the Technion, Faculty of Biology, Auditorium. Please find enclosed the program. Technion Genome Center Single Cell Workshop Participation is free but registration is required. Please register here. For more information, please contact Dr. Tal Katz-Ezov, 04-8295168, talke@technion.ac.il The TGC team    ...

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Save the Date – LSE Open Day – 31.1.19

Dear all, The LS&E Infrastructure invites you to an "Open Day", on January 31st 2019.  Where? Faculty of Biology, Auditorium  When? January 31st 2019 at 09:15  Program as a pdf file available here  In this open day, we will present the various applications available at the LS&E infrastructure center.  After the presentation, there will be a tour in the center's units.   We encourage everyone to come, in particular,  lab managers and new students, so please forward this mail to them.  Registration to this open day, and the tour is needed. Please register here.  Looking forward to seeing you all,  Maayan and the LSE team...

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The LS&E and BCF Workshop on Light Microscopy and Image Processing & Analysis

We are happy to invite you to a joint BCF/LS&E imaging workshop. Day 1: Monday, December 3, 2018 Location: Seminar Room, Mezzanine, Faculty of Medicine Light Microscopy 09:00-12:00 Theoretical Instruction Transmitted light. Fluorescence: Widefield and Confocal Maya Holdengreber Field Application Specialist, BCF  12:00-12:45, Lunch break 12:45-13:45 High End Applications - BCF Introduction to Bioimaging Center - BCF Edith Suss-Toby Director, Bioimaging Center, BCF LSM880 confocal for high resolution high speed & spectral imaging Maya Holdengreber Field Application Specialist, BCF Incucyte – High Throughput Live Cell Analysis System Melia Gurewitz Field Application Specialist, BCF 13:45-14:00, Coffee break 14:00-15:00 High End Applications – LS&E Light Sheet Microscopy for 3D Imaging of live or fixed samples Nitsan Dahan Director, Microscopy & Imaging, LS&E, Technion Incell 2000 for high content imaging and analysis Yael Lupu-Haber Imaging & Bioanalysis specialist, LS&E Image stream  – to see behind the dot Efrat Barak Flow Cytometry Head, LS&E   Day 2: Tuesday, December 4, 2018 Location: Computer Farm, First Floor, Faculty of Biology Image Processing & Analysis 09:00-10:15 The Numbers Behind the Image Yael Lupu-Haber Imaging &...

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November 8th, 2017 – LS&E Infrastructure Center Users Symposium

The LS&E Infrastructure Center happy to the invites You to attend a special LS&E Users Symposium day. Date: Wednesday, Nov 8th, 10:00 o'clock, Location:  Biology faculty auditorium, Technion Speakers: Session I: Yarden Golan, Bea Kaufmann, Moran Hod Marco & Leon Anavy Session II: Mor Goldfeder, Roni Hass, Boris Shneyer & Noa Ben-Asher. Session III: Despina Soteriou, Yulia Fridman & Nitzan Krinsky.   15:30 - End of Symposium This symposium is free of charge, however, registration is required using this link. Looking forward to seeing you all, LSE Infrastructure Center team...

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June 28th, 13:00 – Special seminar on IN Cell Analyzer – New platform to meet your High Content Analysis needs

The LS&E Microscopy core facility the invites You to attend special seminar on "IN Cell Analyzer - New platform to meet your High Content Analysis needs". Date: Wednesday, June 28th, 13:00 o'clock, (45 min's talk) Location:  Biology faculty auditorium, Technion Speaker: Dr. Przemysaw Fleszar, Application Specialist, Cell Analysis, GE Healthcare Title: IN Cell Analyzer - New platform to meet your High Content Analysis needs Abstract: Recent developments in the IN Cell portfolio enable powerful high content analysis (HCA) capabilities on live cell assays, using widefield deconvolution, line scan confocal and transmitted light imaging modalities. These cutting edge hardware platforms use class leading technologies in concert with sensitive and powerful image analysis platforms to give the user the statistical power to address biological questions in cell biology that previously lay out of reach. Results on the microscopic scales ranging from tissues, cell populations down to single cells, investigating molecular organization of individual cells in even the most sensitive...

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April 25th, 10:30 – Seminars on Clearing methodologies for biological samples

The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies. The next seminars will be on "Clearing methodologies for biological samples".   Date: Tuesday, April 25th, 10:30 o'clock, (45 min's talk) Location:  Room 4-17, Emersson Building Speaker: Shlomi Lazar, Ph.D. Division of medical chemistry, The Israel institute for Biological Research (IIBR). Title: Looking inside the organs: a comparative analysis of three methods for establishing optically transparent samples   Abstract: Typical histological study, using sectioning of the tissue, has major limitations in obtaining 3D images of structural components and cells distribution within tissues. Ideally, samples should be imaged at high spatial resolution with minimal sectioning. However, thick tissue imaging is limited mostly because of light scattering. In this study, we compared the efficacy of three recently published clearing protocols (Scale, 3DISCO and Clarity) in generating a transparent thick section which can be subjected to confocal analysis. Brains, ovaries and embryos obtained from transgenic mice expressing enhanced yellow fluorescent protein (eYFP) in...

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