
- Publications
- Microscopy & imaging
- Super Resolution Microscope: Lattice SIM/STED/STORM
- ZEISS- Light Sheet Z7
- Nikon- Spinning Disk Confocal
- LSM 880- Upright confocal with MP laser
- LSM 710 – Inverted confocal
- LSM 700 – Inverted confocal
- LSM 980- Spectral inverted confocal with Airyscan2
- Cytation™ 5 – BioTek
- Leica DMI8 Inverted Fluorescent Microscope
- Olympus Fluorescent Binocular Microscope
- Image analysis & processing
- Flow Cytometry
- Virus room – Emerson Building
Cytation™ 5 – BioTek
Description
Cytation™ 5 systems combine automated digital microscopy, BioSpa incubator for up to 8 multi-dish plates and a robot machine. The microscopy module offers up to 20x magnification in fluorescence, high contrast brightfield, color imaging and phase contrast. Gen5™ software provides complete control over all imaging and data capture with powerful image and data analysis. In addition, the system includes an automated scratcher for live cell imaging of wound healing assay.
Features
- Speed: Bright optics with powerful light source
Fast on-line cell count function
Rapid slide imaging
Automated filter & objective changing - Environmental chamber: Variable temperature control (up to 42°C), CO2 and humidity
- Manual microscope mode
- Preview Scan
- Slide imaging
- Automated image focusing based on laser and/or software
- Transmitted light imaging (Phase)
- Large chip CCD camera
Objectives
Magnitude | NA | Working Distance |
4X | 0.1 | 8.5 mm |
10X | 0.3 | 6.0 mm |
20X | 0.45 | 5.5 mm |
Fluorescence Filters:
The imaging system includes 3 filters covering most popular dyes and fluorescence proteins
• DAPI/Hoechst
• eGFP/FITC/ Alexa Flour 488
• RFP/Cy3/TRITC/DsRed/mOrange/Rhodamine
Applications
The designed-in features and functions enable complex biological techniques to be incorporated with ease into your high content program. Applications could be performed with Cytation™ 5 – BioTek include:
- Compound screening
- Stem cell assays
- Phenotypic profiling
- RNAi screening
- Tissue microarrays
- Whole organism imaging
- Cell migration
- Micronuclei formation
- Co-localization analysis
- Automated slide imaging
- Fluorescent in situ hybridization
- Spheroid studies
- Organelle & protein trafficking
- Morphology analysis
- DNA content analysis
- Apoptosis/cell viability
- Colony counting (e.g. stem cells)
Software:
Gen5 Image+ offers complete imaging control for Cytation 5, and provides image processing and analysis including z-projection, image stitching, and digital phase contrast.
Gen5 Image Prime controls Cytation 5 includes more expansive image processing and analysis: Z-projection, image stitching, digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis