Cytek Aurora is a spectral flow cytometer equipped with four lasers (405, 488, 561, 635 nm). The Aurora’s advanced technology allows the detection of the full emission spectrum, which enables it to distinguish between overlapping dyes such as GFP and FITC or APC and AF 647.

A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates.

In addition the AURORA can functions conventional mode like classical flow cytometer.

Single color controls and autofluorescence extraction are not necessary for every assay.
With SpectroFlo software version 3.3, you can treat the Cytek Aurora™ system like a conventional flow cytometer, looking only at the peak channel for each fluorochrome. This allows users to perform conventional compensation, while also simplifying daily assays where single color controls are not typically collected (e.g. cell cycle analysis).

Features

• Violet detector module (laser ex. – 405 nm): 16 channels unevenly spaced bandwidth from 420-829 nm.
• Blue detector module (laser ex. – 488 nm): 14 channels unevenly spaced bandwidth from 498-829 nm.
• Yellow-Green detector module (laser ex. 561 nm): 10 channels unevenly spaced bandwidth from 567-829 nm.
• Red detector module (laser ex. 635 nm): 8 channels unevenly spaced bandwidth from 652-829 nm.
• Automatic Micro-Sampling System (AMS) – enables analysis directly from 96 multi-well plates (flat, u/v shape).

 

 

48 different fluorophores + FSC & SSC

Sample flow rates:

• low (15µl/min)
• Medium (30µl/min)
• High (60µl/min)

Software: SPECTROFLO®SOFTWARE

• Live unmixing during acquisition
• Developed specifically to streamline assay setup, data acquisition, and file export.
• Autofluorescence extraction
• Raw and Unmixed FCS 3.1 files

 

Experiment design tool: https://spectrum.cytekbio.com/