BD Influx System – Sorter

Description

This Instrument is a flexible and stable stream-in air flow cytometry sorter platform and is capable of high speed and single cell sorting.

The system has 2 laser excitation (488nm and 457nm) and up to 9 fluorescent detectors.

The instrument has the capability of up to six-way sorting into tubes or single cell sorting directly into plates or slides (96, 384 and 1536 wall plates).

A small particle detection adaptor (>100nm) is also available for marine biology, microbiology and environmental applications.

 

Features

 

Laser line/color
(Excitation)
Detector Mirror Filter

457nm

 A 550LP

692/40

 B  488RB

480/40

488 nm

 C  735LP  750LP
 D 655LP 692/40
E 600LP 610/20
 F   550LP

580/30

 G 502LP 530/40
H SSC
 I  FSC

 

 

Specifications and performance

 

Optics 

For each laser, light is shaped by individual optics, and final alignment and focusing are achieved using a dedicated lens that steers light to the stream at different angles. The laser paths are collinear through the steering optics and focusing lens, but intersect the stream at spatially separated points. Fluorescence is collected through a high NA lens and focused on individual mirrored pinholes, reducing crosstalk between lasers.

 

Small Particle Option

The Small Particle Option on the BD Influx system improves forward scatter detection. This option lowers the threshold on size measurements using a special detector with a high NA microscope lens, a pinhole, and a photomultiplier tube (PMT). The pinhole reduces the amount of stray light reaching the detector and prevents saturation of the PMT. Optimized sheath fluid (0.1-micron filtered) lowers the intrinsic noise level, which enables measurement of particles as small as 200 nm.
This resolution makes the Small Particle Option particularly valuable for applications in marine biology, microbiology, and environmental biology. The Small Particle Option also allows researchers to detect fluorescence in the forward direction. This capability is used in applications such as chlorophyll detection.

 

Electronics

The BD Influx system uses parallel electronics to reach a throughput rate of 200,000 events per second, independent of the number of parameters. For high sensitivity and low noise, the BD Influx system uses analog electronics in the first stage of signal amplification. The output of the analog peak and hold circuitry is digitized using 16-bit ADCs and placed on a patented parallel processing digital bus. The bus handles real-time compensation as well as other digitized signals, including area and width of up to eight additional pulses. A variety of sort modes, optimized to favor purity, yield, or count accuracy, is standard. A custom mode allows researchers to optimize those settings for a specific application.

 

Nozzle selection and design

An expanded set of nozzles lets users sort a wide range of particle sizes. Four nozzle sizes are supported: 70, 86, 100, 140 and 200 microns.

 

Working policy

Users who wish to work on the Influx system must contact Dr. Efrat Barak before ordering this system.