- LSE Center Publications
- Microscopy & imaging
- Super Resolution Microscope: Lattice SIM/STED/STORM
- Zeiss- Light Sheet Z7
- Nikon- Spinning Disk Confocal
- LSM 880- Upright confocal with MP laser
- LSM 710 – Inverted confocal
- LSM 700 – Inverted confocal
- LSM 980- Inverted confocal with Airyscan2
- Cytation™ 5 – BioTek
- Leica DMI8 Inverted Fluorescent Microscope
- Olympus Fluorescent Binocular Microscope
- Image analysis & processing
- Flow Cytometry
- Virus room – Emerson Building
BD LSR-II SORP Analyzer
The BD LSR-II SORP is an analyzer flow cytometer equipped with 4 lasers.
The cytometer allows detection of 15 parameters simultaneously (15 colors plus forward scatter and side scatter).
The LSR-II SORP offers high acquisition speeds, full inter- and intra-laser compensation matrix, and multiple thresholds on all lasers. Digital pulse sampling allows the measurement of Height, Area and Width from all parameters simultaneously.
Features
- Optical System: Patented BD OctagonTM and TrigonTM technology. A quartz cuvette is coupled to the emission lens by refractive index-matching optical gel for optimum collection efficiency.
- laser excitation and filters (see LSR-II configuration in the related links):
| Laser line/color (Excitation) |
Detector | Emission Filter (nm) | Example of available markers |
|
Violet (405nm)
|
A | 780 / 60 – 740 | BV750, BV785 |
| B | 705 / 70 – 685LP |
BV711 , BV650 |
|
| C | 610 / 20 – 595LP | BV605 | |
| D | 525 / 50 – 505LP | BV510, Zombie Aqua | |
| E | 450 / 50 | Pacific Blue, BV421, DAPI, Hoechst, CFP | |
|
Blue (488nm)
|
A | 710 / 50 – 685LP | PerCP, PerCP-Cy5-5, 7-AAD |
| B | 525 / 50 – 505LP | GFP, AF488, YFP | |
| C | 488 / 10 | SSC | |
| D | FSC | ||
|
Yellow/green (561nm)
|
A | 780 / 60 – 740LP | PE-Cy7 |
| B | 710 / 50 – 685LP |
PE-Cy5.5 |
|
| C | 660 / 20 – 635LP |
PE-Cy5 |
|
| D | 610 / 20 – 600LP |
mCherry, PI |
|
| E | 582 / 15 -570LP |
DsRed, PE |
|
|
Red (640nm)
|
A | 780 / 60 – 740LP | APC- cy7 |
| B | 710 / 50 – 685LP | Vio Bright R720, AF700 | |
| C | 670 / 30 | AF647, APC |
-
High Throughput System (HTS) – enables analysis directly from 96 multi-well plate. The workstation is managed by FACS-Diva 6.1 software.
Additional available filters:
| Mirror | 450LP | 5005LP | 530LP | 635LP | 685LP | 735LP | ||
| Band pass | 405/20 | 525/50 | 530/30 | 585/42 | 610/20 | 670/14 | 675/20 | 695/40 |
Acquisition Rate: >20000 events per second
Fluorescence Sensitivity: Detection limit is <200 molecules of equivalent soluble fluorochrome (MESF).
Fluorescence Resolution: Coefficient of variation (CV) of FL-Area is <3%, determined by full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN Quality Control Kit).
Fluorescence Linearity: Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05, detected with 488nm laser.
Forward and Side Scatter Sensitivity: Sensitivity enables the separation of fixed platelets from noise.
Signal Processing
- Digital electronics with 10 MHz sampling frequency
- 18-bit data acquisition for area signals
- Inter- and intra-beam compensation on-line and post-acquisition off-line
- Height, Width, and Area measurement available for all parameters
- Time parameter available for kinetic studies (Calcium)
- Ratio measurements between any two parameters from any laser
Software
- BD FACSDiVaTM Software Version 6.1
- All data are saved linearly
- Complex hierarchical gating for multicolor applications
- Automatic compensation of all parameters after acquisition
- Complete set of statistics for all analysis
Applications
Analysis of cells, yeast, bacteria and beads
- Multi-color analysis
- HTS system
- Calcium Flux
- DNA analysis
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