ISU > Services & Units > Microscopy & imaging > LSM 880- Upright confocal with MP laser

LSM 880- Upright confocal with MP laser

Description

The LSM 880 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized Axio Imager Z1 microscope.

Features

Laser options:

The system includes 5 visible lasers and an IR-multi photon laser:

Diode laser 405 nm (10mW)
Multiline Argon laser with excitations at: 458, 488, 514nm (25mW)
Laser He-Ne 543 nm (10mW)
Laser He-Ne 594 nm (10mW)
HeNe laser, with excitation line at: 633 nm (5mW)

– Ti-Sapphire Multi-photon laser with excitation line varied from 690-1040 nm (4.2 W at 800 nm)

Objectives:

Objective	Plan-Apochromat 10x	C-Apochromat	LD LCI Plan-Apochromat	Objective Plan-Apochromat- DIC
Magnitude	10x	10x	X25	X63
Serial #	420640-9800-000	421747-9900-000	420852-9870-000	420782-9900-799
NA	0.3	0.45	0.8	1.4
Immersion	dry	Water	Water ± cover slip / Glycerol ± cover slip / Oil (correction ring)	Oil

Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.

Detection: 3 individual fluorescent channels: 2 PMT detectors and 1 GaAsp detector for spectral imaging. A variety of available emission filters offers imaging of wide fluorochromes from the UV to the IR spectral region 1 transmitted light channel allowing superimposing fluorescence images on bright filed, DIC images.

2-photon application: the TiSapphier laser allows 2-photon excitation which permits dipper penetration into tissues and samples.

Acquisition and analysis: acquisition is performed using the ZEN software which provides a wide range of image processing functions: 2D/3D, projection, reconstructing, co localization, intensity measurements and more.
An additional software license for offline analysis is available on the workstation.
Files generated by the 880 LSM software can be loaded by the ZEN . Using the ZEN lite one can export specific files to any other required form: JPEG, AVI, etc.