Microscopy & imaging

The Microscopy core facility center contains: Multi-modality super resolutions microscope (SIM/STED/STORM), Light Sheet microscope, 4 confocal microscopes, High Content imaging systems (Cytation 5 & incell 2000), 2 inverted fluorescent light microscopes and 3 fluorescent binoculars. Additionally, there are work stations containing image analysis software, including Deconvolution software, time-lapse, 3-D reconstruction and other software.

All users receive full training on a specific instrument prior to independent work.

Workshops and hands-on sessions are organized to provide researchers with training in fluorescent and confocal microscopy. Additionally, a forum is managed for open discussions relating to new technologies, and sharing of ideas and personal experiences with the various systems.

The center contains the following microscopes:

High end microscopes:

• Elyra 7 eLS  – Multi Modality Super Resolution (SR) microscope that combined lattice SIM, 2D-STED and STOTM/PALM methods for SR, this system is designed for live-cell imaging.
• Light Sheet Fluorescent Microscope– Zeiss Z7, with dual sided illumination, 2 cameras and full incubation. An analysis computer is available for the Light Sheet data analysis.

Confocal Microscopes:

• Spinning Disk Confocal microscope from Nikon with Yokogawa CSU-W1 Confocal Scanner Unit with dual sCMOS Prime BSI camera from photometrics, this system is designed for fast imaging and for long live-cell imaging.
• Confocal Zeiss LSM 980, an inverted confocal with 4 laser-lines, 2 PMT detectors, 32 ch GaAsp array detectors and Ariyscan2 (super-resolution) detector. Included full incubation, the system is designed for live cell imaging.
• Confocal Zeiss LSM 880, an upright microscope with 5 lasers, including a multi-photon laser and GaAsp and NDD detectors.
• Confocal Zeiss LSM 710, an inverted confocal with 6 laser-lines, 4 detectors and full incubation, dedicated for live cell imaging.
• Confocal Zeiss LSM 700, an inverted microscope with 4 lasers suitable for live cell imaging.

High Content Analysis system:

• Cytation™ 5 system combines automated digital microscopy, BioSpa incubator for up to 8 multi-dish plates and a robot machine.

Fluorescent Microscopes:

• Inverted Leica DMI8– inverted fluorescent microscope.

Fluorescent Binocular:

• Olympus Fluorescent Binocular Microscope

All users must download the User Statement and center policy and sign it before starting to work (See the Ordering section on this site, sub heading: User Statement and center Policy).

Microscopy Team: