Description

Multi Modality Super Resolution (SR) microscope that combines lattice SIM, 2D-STED and STOTM/PALM methods for SR, this system is designed for live-cell imaging.
The Elyra 7 eLS is a flexible platform featuring unique lattice structured illumination (SIM^2) for fast and gentle 3D super-resolution on live samples as well as Single Molecule Super-resolution imaging. Lattice SIM^2 provides optical sectioning and a doubling of diffraction-limited resolution in 3D (60 nm in xy and 150 nm in z). SMLM part allows for resolutions in the typical range of 20-30 nm laterally and 50-80 nm axially. The system features groundbreaking light efficiency and allows for gentle live cell imaging at speeds of dozens of frames per second over time. It includes state-of-the-art sCMOS cameras for large field  of view imaging, SIM- and SMLM optimized optics, focus drift compensation control and
full incubation.
The STEDYCON is a laser scanning confocal microscope and STED nanoscope with a resolution down to 30 nm. It has a super-intuitive user interface.

 

Features

SYSTEM SPECIFICATIONS:

Super Resolution (SR) imaging: Lattice SIM^2 (Zeiss), STORM (Zeiss) and STED (Abberior)
Confocal imaging : StedyCon (Abberior- confocal)
Z Stack imaging: Apotom (Zeiss)
TIRF imaging:
Stand: Zeiss Axio Observer 7 SR RP stand for ELYRA 7
XY-stage: motorized
Z – Z PIEZO WSB 500 um range
Definite Focus 2 (Zeiss)
StedyFocus (Abberior)
HXP fluorescence  illumination
Equipped for live imaging (temperature and CO2 incubation)
Software: ZEN

 

LASER OPTIONS:

The Zeiss system includes 4 visible solid state lasers:

– UV diode laser – 405 nm (50mW)
– Blue diode laser 488  (500mW)
– Green diode laser – 561 nm (500mW)
– Red diode laser – 642 nm (500mW)

 

The Abberrior system includes 4 visible solid state lasers:

– UV diode laser – 405 nm (continues)
– Blue diode laser 488  (pulse)
– Green diode laser – 561 nm (pulse)
– Red diode laser – 640 nm (pulse)

STED laser depletion: 775nm (pulsed)

 

CAMERA SPECIFICATIONS:
Model:  Two pco.edge sCMOS (version 4.2 CL HS)
Effective number of pixels: 2048 x 2048
Pixel size: 6.5µm x 6.5µm
Effective Area: 13.312mm x 13.312mm
Exposure times from 100 µs to 20 s
Dynamic range: 16 bit
Max frame rate: 100 fps @ full frame (CL/CLHS)
40 fps @ full frame (USB)
Quantum efficiency (peak): > 82% (@590nm)
Read out noise: extreme low readout noise of 0.8 e.

 

 

Catalog #	420340-9901-000	420650-9902-000	420762-9900-000	421787-9970-799	420782-9900-720	420780-9971-000	420792-9800-720
Magnitude	10x	20x	40x	63x	63x	63x	100x
Type	Plan-Apochromat	Plan-Apochromat	Plan-Apochromat	C-Apochromat	Plan-Apochromat	alpha Plan-Apochromat	alpha Plan-Apochromat
NA	0.3	0.8	1.4	1.2	1.4	1.46	1.46
Immersion	Dry DIC	Dry DIC	Oil DIC	Water DIC	Oil DIC	Oil DIC	Oil DIC
Imaging Application	WF/FL	WF/FL/ Apotome	WF/FL/ Apotome	WF/FL/ Apotome/ Lattice SIM	WF/FL/ Apotome/ Lattice SIM	WF/FL/ Apotome/ Lattice SIM/ STED/TIRF	WF/FL /Apotome/ STORM/PALM/ STED/TIRF

 

CAMERA FILTERS:

 

Beam Splitter	LP 560		BP490-560/ LP640
CAMERA	CAM1	CAM2	CAM1	CAM2
Detection	BP 570-620	BP 420-480	BP 495-550	BP 420-480
Detection	LP 655	BP 495-550	LP 655	BP 570-630
Detection	—	—	—	LP 740

 

Acquisition & Analysis:

• Acquisition is performed using the ZEN black software which provides wide range of image processing functions: 2D/3D, projection, reconstructing, co localization, intensity measurements and more. The flexible secondary dichroic beam splitter makes this system fully capable of acquiring super resolution and performing dual camera imaging. In addition, Elyra 7 with Lattice SIM^2 takes you beyond the diffraction limit of conventional microscopy to image your samples with super-resolution. Elyra 7 is the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors. Imaging with incredibly high speed – at 255 fps. Elyra 7 combine Lattice SIM^2 with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.
• The STEDYCON software has a user friendly GUI that required only several minutes of training to acquire STED and confocal images.

Applications

The system enables amongst many others, the following applications:

• Super-Resolution Imaging: 
  Lattice Structure Illumination Microscopy (Lattice SIM^2)
  STimulated Emission Depletion (STED) Microscopy :2D STED
  Single Molecule Localization Microscopy (SMLM): PALM/dSTORM/PAINT
• Section Imaging (Z stack):
  Apotome -Zeiss
  Confocal -Abberior
• Total Internal Reflection Fluorescence (TIRF) Microscopy
• Fast Image Acquisition for Live Cell Imaging and Time-Lapse studies
• Z-Stacks for localization of cells in living tissues and 3-D reconstruction
• Super-resolution localization of sub-cellular compartments and quantities co-localization
• FRET – fluorescence resonance energy transfer
• FRAP – fluorescence recovery after photobleacing in a super-resolution microscopy
• PhotoActivation and PhotoManipulation in a super-resolution microscopy

* Mark and find feature – The motorized stage allows marking specific coordinates using small magnifications or tiling of large scanning areas for further detailed scanning.