- LSE Center Publications
- Microscopy & imaging
- Super Resolution Microscope: Lattice SIM/STED/STORM
- Zeiss- Light Sheet Z7
- Nikon- Spinning Disk Confocal
- LSM 880- Upright confocal with MP laser
- LSM 710 – Inverted confocal
- LSM 700 – Inverted confocal
- LSM 980- Inverted confocal with Airyscan2
- Cytation™ 5 – BioTek
- Leica DMI8 Inverted Fluorescent Microscope
- Olympus Fluorescent Binocular Microscope
- Image analysis & processing
- Flow Cytometry
- Virus room – Emerson Building
LSM 880- Upright confocal with MP laser
Description
The LSM 880 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized Axio Imager Z1 microscope.
Features
- Laser options:
The system includes 5 visible lasers and an IR-multi photon laser:
- Diode laser 405 nm (10mW)
- Multiline Argon laser with excitations at: 458, 488, 514nm (25mW)
- Laser He-Ne 543 nm (10mW)
- Laser He-Ne 594 nm (10mW)
- HeNe laser, with excitation line at: 633 nm (5mW)
– Ti-Sapphire Multi-photon laser with excitation line varied from 690-1040 nm (4.2 W at 800 nm)
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Objectives:
Objective | Plan-Apochromat 10x | C-Apochromat | LD LCI Plan-Apochromat | Objective Plan-Apochromat- DIC |
Magnitude | 10x | 10x | X25 | X63 |
Serial # | 420640-9800-000 | 421747-9900-000 | 420852-9870-000 | 420782-9900-799 |
NA | 0.3 | 0.45 | 0.8 | 1.4 |
Immersion | dry | Water | Water ± cover slip / Glycerol ± cover slip / Oil (correction ring) |
Oil |
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Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
- Detection: 3 individual fluorescent channels: 2 PMT detectors and 1 GaAsp detector for spectral imaging. A variety of available emission filters offers imaging of wide fluorochromes from the UV to the IR spectral region 1 transmitted light channel allowing superimposing fluorescence images on bright filed, DIC images.
- 2-photon application: the TiSapphier laser allows 2-photon excitation which permits dipper penetration into tissues and samples.
- Acquisition and analysis: acquisition is performed using the ZEN software which provides a wide range of image processing functions: 2D/3D, projection, reconstructing, co localization, intensity measurements and more.
An additional software license for offline analysis is available on the workstation.
Files generated by the 880 LSM software can be loaded by the ZEN . Using the ZEN lite one can export specific files to any other required form: JPEG, AVI, etc.
Applications
Multi channel fluorescence imaging
3-D reconstruction
Quantitative co-localization
Spectral imaging
Time-series
SHG – Second Harmonic Generation Imaging
FRET – fluorescence resonance energy transfer
FRAP – fluorescence recovery after photo-bleaching
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