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LSM 880- Upright confocal with MP laser
Description
The LSM 880 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized Axio Imager Z1 microscope.
Features
- Laser options:
The system includes 5 visible lasers and an IR-multi photon laser:
- Diode laser 405 nm (10mW)
- Multiline Argon laser with excitations at: 458, 488, 514nm (25mW)
- Laser He-Ne 543 nm (10mW)
- Laser He-Ne 594 nm (10mW)
- HeNe laser, with excitation line at: 633 nm (5mW)
Ti-Sapphire Multi-photon laser with excitation line varied from 690-1040 nm (4.2 W at 800 nm)
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Objectives:
Objective | Plan-Apochromat | C-Apochromat | Plan-Apochromat | LD LCI Plan-Apochromat | Plan-Apochromat- DIC |
Magnitude | 10x | 10x | 20x | X25 | X63 |
Serial # | 420640-9800-000 | 421747-9900-000 | 420650-9902-000 | 420852-9870-000 | 420782-9900-799 |
NA | 0.3 | 0.45 | 0.8 | 0.8 | 1.4 |
Immersion | dry | Water | dry | Water ± cover slip / Glycerol ± cover slip / Oil (correction ring) |
Oil |
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Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
- Detection: Three individual fluorescent channels: two PMT detectors and one GaAsp detector for spectral imaging. A variety of available emission filters offers imaging of wide fluorochromes from the UV to the IR spectral region and one transmitted light channel allowing superimposing fluorescence images on bright filed, DIC images.
- 2-photon application: the TiSapphier laser allows 2-photon excitation which permits dipper penetration into tissues and samples.
Applications
Multi channel fluorescence imaging
3-D reconstruction
Quantitative co-localization
Spectral imaging
Time-series
SHG – Second Harmonic Generation Imaging
FRET – fluorescence resonance energy transfer
FRAP – fluorescence recovery after photo-bleaching
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