LSM 880- Upright confocal with MP laser

Description

The LSM 880 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized Axio Imager Z1 microscope.

 

Features

  • Laser options:

The system includes 5 visible lasers and an IR-multi photon laser:

  • Diode laser 405 nm (10mW)
  • Multiline Argon laser with excitations at: 458, 488, 514nm (25mW)
  • Laser He-Ne 543 nm (10mW)
  • Laser He-Ne 594 nm (10mW)
  • HeNe laser, with excitation line at: 633 nm (5mW)

Ti-Sapphire Multi-photon laser with excitation line varied from 690-1040 nm (4.2 W at 800 nm)

  • Objectives:
Objective Plan-Apochromat C-Apochromat Plan-Apochromat LD LCI Plan-Apochromat Plan-Apochromat- DIC
Magnitude 10x 10x 20x X25 X63
Serial # 420640-9800-000 421747-9900-000 420650-9902-000 420852-9870-000 420782-9900-799
NA 0.3 0.45 0.8 0.8 1.4
Immersion dry Water dry Water ± cover
slip / Glycerol ±
cover slip / Oil
(correction ring)
Oil

 

  • Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
  • Detection: Three individual fluorescent channels: two PMT detectors and one GaAsp detector for spectral imaging. A variety of available emission filters offers imaging of wide fluorochromes from the UV to the IR spectral region and one transmitted light channel allowing superimposing fluorescence images on bright filed, DIC images.
  • 2-photon application: the TiSapphier laser allows 2-photon excitation which permits dipper penetration into tissues and samples.

Applications

Multi channel fluorescence imaging

3-D reconstruction

Quantitative co-localization

Spectral imaging

Time-series

SHG – Second Harmonic Generation Imaging

FRET – fluorescence resonance energy transfer

FRAP – fluorescence recovery after photo-bleaching

Mark and Find

 

Releted Documents

Spectral Imaging

Introduction To LSM

Meta Emission Fingerprinting

Multi Photon Microscopy

Multi Photon Dye Chart

Releted Links

Download ZEN Lite

Know How – Laser Scanning System (Zeiss)

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