LSM 710 – Inverted confocal

Description

The LSM 710 laser scanning confocal microscope from Zeiss is a spectral imaging confocal system designed for live-cell imaging. It is hooked to an inverted fully motorized Axio Observer Z1 microscope.

Features

• Laser options:

The system includes 4 visible solid state lasers:

– UV laser – 405 nm (10mW)

– Argon multi line laser – 458, 488 and 514 nm (30mW)

– Green laser – 543 nm (10mW)

– Red laser – 639 nm (5mW)

• Objectives:

• Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
• Detection: The scanning module features a confocal beam path with two channels and continuously variable secondary dichroic beam splitter for free selection of emission bands; 2 symmetric galvanometer mirrors for high-precision beam positioning; One motorized and continuously variable high precision pinhole; Two PMTs (photomultipliers) detectors and Two highly sensitive GaAsp (Gallium arsenide phosphide) detectors. In addition, the system provides transmission images via the Transmission Photomultiplier.

• Incubation: This microscope has an incubation chamber for live cell imaging for control over CO2, temperature and humidity.

Applications

The system enables, amongst many others, the following applications: Photoactivation and Photobleaching experiments with up to 99 freely definable ROI´s,

* Fast Image Acquisition for Live Cell Imaging and Time-Lapse studies,

* Z-Stacks for localization of cells in living tissues and 3-D reconstruction

* High-resolution localization of sub-cellular compartments and quantities co-localization

* Spectral imaging

* FRET – fluorescence resonance energy transfer

* Mark and find feature – The motorized stage allows marking specific coordinates using small magnifications or tiling of large scanning areas for further detailed scanning.

Related Links

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