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- LSM 700 – Inverted confocal
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LSM 700 – Inverted confocal
Description
The LSM 700 laser scanning confocal microscope from Zeiss is hooked to an inverted fully motorized Axio Observer Z1 microscope.
Features
- Laser options:
The system includes 4 visible solid state lasers:
– UV laser – 405 nm (5mW)
– Blue laser – 488 nm (10mW)
– Green laser – 555 nm (10mW)
– Red laser – 639 nm (5mW)
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Objectives:
| Magnitude | X10 | X20 | X40 | X40 | X63 |
| Type | EC Plan- Neofluar |
Plan-Apochromat | LD Plan- Neofluar |
EC Plan Neofluar |
Plan Apochromat |
| NA | 0.3 | 0.8 | 0.6 | 1.3 | 1.4 |
| Immersion | dry | dry | Air (correction ring) |
Oil | Oil DIC |
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Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
- Detection: The scanning module features a confocal beam path with 2 channels and continuously variable secondary dichroic beam splitter for free selection of emission bands; 2 symmetric galvanometer mirrors for high-precision beam positioning; 1 motorized and continuously variable high precision pinhole; Two PMT detectors (photomultipliers).In addition, the system provides transmission images via the Transmission Photomultiplier.
- Incubation: This microscope has a small incubation chamber for live cell imaging for control over CO2, temperature and humidity.
Applications
The system enables, amongst many others, the following applications: Photoactivation and Photobleaching experiments with up to 99 freely definable ROI´s,
* Image Acquisition for Live Cell Imaging and Time-Lapse studies,
* Z-Stacks for localization of cells in living tissues and 3D reconstruction
* High-resolution localization of sub-cellular compartments and quantities co-localization
* FRET – fluorescence resonance energy transfer
* Mark and find feature – The motorized stage allows marking specific coordinates using small magnifications.
Related Documents
Introduction To LAM And Principals
Related Links
ZEN Software – Efficient Navigation