News & Events
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies.
The next seminar will be on “Insights into super-resolution – methodologies and applications of super-resolution microscopy “.
Date: Tuesday, March 21st, 10:00 o’clock
Please keep note that the seminar will be 30 minutes earlier than the original plan.
Location: Auditorium , Faculty of Biology
Speaker: Avi Jacob, Ph.D.
Head of Light Microscopy facility, Bar-Ilan University.
Microscopy-based methods for breaking diffraction limited resolution are collectively known as super-resolution (SR). There are several methods which can be divided into two main approaches: deterministic and stochastic. Stochastic methods include all localization techniques such as STORM and deterministic methods include the optical approaches such as STED. STED (STimulated Emission Depletion) is a mature technology, yet can require optimization to reach ~50nm resolution. Recently, companies have begun to offer “hardware-optimization + software based SR”, whereby a measure of SR is achieved by automatically optimizing acquisition and then submitting to deconvolution. Each has their advantages.
Model systems need to be appropriate for SR, such as sub organelle structures, nuclear pores or fine membrane studies. Also, care must be taken to ensure that SR is technically possible taking into account the specific SR method used. For example, with STED, it’s possible the antibody-secondary-antibody tree could be larger than the airy disk. A balance must be struck between the more time consuming, yet higher resolution STED, and the faster “middle of the way” approach that can be universally used.
Light refreshment will be served before the seminar
Looking forward to seeing you all,
LSE Microscopy team
The LS&E Microscopy core facility the invites You to attend special seminars in Advanced microscopy application and Methodologies.
The first seminar will be on “Dynamic Imaging of Living Samples”.
Date: Tuesday, February 28th, 10:30 o’clock
Location: Auditorium , Faculty of Biology
Speaker: Douglas Lutz, Ph.D.
Advanced Imaging Applications Specialist Getter Group Ltd- Bio Med Scientific Div.
Abstract: A variety of modern imaging techniques will be presented that are especially well suited to the investigation of living samples. We will discuss the wide variety of light microscopic imaging methodologies available, how one goes about choosing the best method for different sample types, optical considerations that go into the decision making process, and how to maintain and evaluate “normal” sample physiology during the observation time frame.
The LS&E Microscopy core is delighted to announce on seminars series on advanced microscopy applications and methodologies for Life Sciences Imaging taking place the biology faculty over the coming months. These seminars are designed as a program of short talks to enrich your knowledge on Live cell imaging, Clearing methodologies , Super-resolution and Image analysis aimed to help you optimize your microscopy imaging workflow. These seminars are free of charge, however, registration in event form is required. Looking forward to seeing you all, LS&E microscopy core team.
The Life Science and Engineering (LS&E) Infrastructure Center is happy to invite you to a special symposium on flow cytometry on Tuesday, March 7, 2017.
The symposium will be held in the Auditorium of Faculty of Biotechnology and Food Engineering.
On this one day event we will introduce the basics of flow cytometry and how it applies to various research areas.
Selected talks by researchers from different faculties at the Technion,
whose work will give you a taste of what can be achieved at our LS&E Flow Cytometry Unit, will be presented.
09:15-09:30 – Coffee and gathering
09:30-11:00 Session I – Flow cytometry as a research tool
Liat Bilinsky, Application Specialist, BD Biosciences:
Introduction to Flow Cytometry , Instrument components – Fluidics, Optics, Electronics
Instrument settings – How to build an experiment from scratch
11:00-11:20 Coffee break
11:20-12:15 – Session II – Flow cytometry applied– selected talks by Technion’s students
Nitsan Fourier, Ben-Zion Levi’s lab, Faculty of Biotechnology & Food Engineering:
shRNA library screen identifies Mafk as repressor of IRF8 in non-hematopoetic cells
Samah Awwad, Nabieh Ayoub’s lab, Faculty of Biology:
NELF-E is recruited to DNA double-strand break sites to promote transcription repression and repair
Tamar Hashimshony, single cell genomics application specialist at the TGC,LS&E Infrastructure.
FACS & NGS – single cell whole transcriptome RNA analysis using CEL-Seq
12:15-13:00 – Lunch break
13:00-13:50 Session II – continued
Zvi Yaari, Avi Schroeder’s lab, The Chemical Engineering Faculty:
Theranostic Barcoded Nanoparticles for Personalized Cancer Medicine
Noa Katz, Roee Amit’s lab, Faculty of Biotechnology & Food Engineering:
A Synthetic Biology Approach for Screening RBP Binding Sites
Andrey Rozenberg, Oded Beja’s lab, Faculty of Biology:
Who infects whom in bacteria-phage communities: an approach utilizing cell encapsulation
13:50-14:00 – Coffee break
14:00-15:00 Session III – Advanced multi parametric flow cytometry
David Merkel Golan, General Manager, Merkel Technologies Ltd:
Multi-parametric Flow Cytometry
The LS&E microscopy unit is happy to announce that HIVE technology is available for IMARIS and Light-sheet users. HIVE is a high speed centralized data repository that removes the need to constantly move or duplicate data sets in processing and analysis workflows The HIVE is a modular platform (computation & storage server) optimized for image processing and data handling. The HIVE designed for the pragmatic, biology-focused scientist, it boosts productivity in the LS&E microscopy unit .Its modular design integrates high speed processing, visualization, remote access, flexibility, data security, scalability and ease of use in one unit.