News & Events

25/06/2017

July 7th, 10:00 – Special seminar on Imaging Flow Cytometry (IFC) using the Image Stream

The LS&E Flow Cytometry unit the invites You to attend special seminar on “Imaging Flow Cytometry (IFC) using the Image Stream“.

Date: Wednesday, July 7th, 10:00 o’clock,

Location:  Biology faculty auditorium, Technion

Speaker: Dr. Yael Lupu-Haber, LSE application specialist for the Image Stream

Title: Imaging Flow Cytometry (IFC) using the Image Stream

Abstract:

IFC combines speed, sample size and phenotyping abilities of flow cytometry with the morphological analysis ability of microscopy in a single instrument platform. It allows statistical analysis of cellular morphology, fluorescent intensity and texture.
IFC applications include:
Cell signaling, Internalization ,Co-localization ,Microbiology ,Cell cycle ,Apoptosis ,Shape change ,Cell-cell interactions ,Oceanography.

Looking forward to seeing you all,

LSE Flow Cytometry team

June 28th, 13:00 – Special seminar on IN Cell Analyzer – New platform to meet your High Content Analysis needs

The LS&E Microscopy core facility the invites You to attend special seminar on “IN Cell Analyzer – New platform to meet your High Content Analysis needs“.

Date: Wednesday, June 28th, 13:00 o’clock, (45 min’s talk)

Location:  Biology faculty auditorium, Technion

Speaker: Dr. Przemysaw Fleszar, Application Specialist, Cell Analysis, GE Healthcare

Title: IN Cell Analyzer – New platform to meet your High Content Analysis needs

Abstract:

Recent developments in the IN Cell portfolio enable powerful high content analysis (HCA) capabilities on live cell assays, using widefield deconvolution, line scan confocal and transmitted light imaging modalities. These cutting edge hardware platforms use class leading technologies in concert with sensitive and powerful image analysis platforms to give the user the statistical power to address biological questions in cell biology that previously lay out of reach. Results on the microscopic scales ranging from tissues, cell populations down to single cells, investigating molecular organization of individual cells in even the most sensitive of live samples.
We will present GE Healthcare’s latest advances in high content imaging technologies, integrated into the new IN Cell Analyzer platforms and our new easy-to-use IN Carta analysis software package. During the session, we will present how to automate image acquisition processes and how to quickly and confidently extract multi-parameter data from these images, regardless of prior experience levels.

Looking forward to seeing you all,

LSE Microscopy team

18/04/2017

April 25th, 10:30 – Seminars on Clearing methodologies for biological samples

The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies.

The next seminars will be on “Clearing methodologies for biological samples“.

 

Date: Tuesday, April 25th, 10:30 o’clock, (45 min’s talk)

Location:  Room 4-17, Emersson Building

Speaker: Shlomi Lazar, Ph.D.

Division of medical chemistry, The Israel institute for Biological Research (IIBR).

Title: Looking inside the organs: a comparative analysis of three methods for establishing optically transparent samples

 

Abstract:

Typical histological study, using sectioning of the tissue, has major limitations in obtaining 3D images of structural components and cells distribution within tissues. Ideally, samples should be imaged at high spatial resolution with minimal sectioning. However, thick tissue imaging is limited mostly because of light scattering. In this study, we compared the efficacy of three recently published clearing protocols (Scale, 3DISCO and Clarity) in generating a transparent thick section which can be subjected to confocal analysis.
Brains, ovaries and embryos obtained from transgenic mice expressing enhanced yellow fluorescent protein (eYFP) in specific cells or wild type mice, were collected and cut to thick sections (2-3mm). Some slices were first fluorescently immunolabeled for various markers. All sections were processed in one or more of the following protocols: (a) The Scale protocol was based on aqueous reagent that renders biological samples optically transparent and completely preserves fluorescent signals in the clarified structures; (b) 3DISCO (3D imaging of solvent cleared organ) was based on organic solvents that dissolve the lipid structure in the tissue and (c) The Clarity protocol was based on hydrogel tissue embedding, lipid extraction in aqueous solution, and clearing. All sections were subjected to confocal analysis. Data acquired was processed using ImageJ software.
We successfully optimized immunofluorescence labeling protocol for floating thick sections (2-3mm). Evaluation of the clear tissues produced by each of the three methods showed that Scale protocol resulted in fragile and only slightly cleared sections. 3DISCO method demonstrated transparent slices but a degraded fluorescent signal over time (1 day half-life). Thick slices processed by Clarity protocol were nicely transparent, maintained their fluorescence intensity, but were greatly more expensive. A combination of Clarity and Scale protocols replacing the high cost clarity reagent with Scale solution, improved the outcome of Scale protocol and preserved the required fluorescence intensity. All slices were successfully analyzed using confocal microscope and no further sectioning was needed.
A combined protocol of Clarity and Scale provide a low cost and available way to generate transparent organs. This enabled detailed analysis of structural and molecular information from thick organ samples.

 

 

Date: Tuesday, April 25th, 11:30 o’clock, (20 min’s talk)
Location:  Room 4-17, Emersson Building
Speaker: Nadav Yayon, M. Sc
PhD student at Prof. Hermona Soreq’s lab, The Edmond and Lily Safra Center of Brain Science. The Hebrew University of Jerusalem
Title: Extracting micro-scale features from macro-scale imaging of iDISCO brains

 

Abstract:
Recent technological advancements enable rendering samples transparent and imaging huge areas of tissue rapidly and in high resolution. However, while these technologies produce beautiful images and 3D movies, extracting quantitative information from such experimental samples is confronted with significant difficulties, both due to sample preparation procedures and because of imaging and analysis constraints. In my talk, I will share my personal experience of using the iDISCO clearing approach and Light-sheet imaging, and will describe our attempts to accurately extract 3D information to identify the location, density and morphology of cortical interneurons in the mouse cortex.

 

Light refreshment will be served before the seminar

Registration below is free of charge but required due to limited place. 

Looking forward to seeing you all,

LSE Microscopy team

Microscopy seminar series
15/03/2017

March 21st, 10:00 – Seminar on Insights into super-resolution

The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies.

The next seminar will be on “Insights into super-resolutionmethodologies and applications of super-resolution microscopy “.

Date: Tuesday, March 21st, 10:00 o’clock

Please keep note that the seminar will be 30 minutes earlier than the original plan.

Location: Auditorium , Faculty of Biology

Speaker: Avi Jacob, Ph.D.

Head of Light Microscopy facility, Bar-Ilan University.

 

Abstract:

Microscopy-based methods for breaking diffraction limited resolution are collectively known as super-resolution (SR). There are several methods which can be divided into two main approaches: deterministic and stochastic. Stochastic methods include all localization techniques such as STORM and deterministic methods include the optical approaches such as STED. STED (STimulated Emission Depletion) is a mature technology, yet can require optimization to reach ~50nm resolution. Recently, companies have begun to offer “hardware-optimization + software based SR”, whereby a measure of SR is achieved by automatically optimizing acquisition and then submitting to deconvolution. Each has their advantages.
Model systems need to be appropriate for SR, such as sub organelle structures, nuclear pores or fine membrane studies. Also, care must be taken to ensure that SR is technically possible taking into account the specific SR method used. For example, with STED, it’s possible the antibody-secondary-antibody tree could be larger than the airy disk. A balance must be struck between the more time consuming, yet higher resolution STED, and the faster “middle of the way” approach that can be universally used.

Light refreshment will be served before the seminar

Looking forward to seeing you all,

LSE Microscopy team

16/02/2017

Seminar series on advanced microscopy applications and methodologies

Microscopy seminar series

 

The LS&E Microscopy core is delighted to announce on seminars series on advanced microscopy applications and methodologies for Life Sciences Imaging taking place the biology faculty over the coming months. These seminars are designed as a program of short talks to enrich your knowledge on Live cell imaging, Clearing methodologies , Super-resolution and Image analysis aimed to help you optimize your microscopy imaging workflow. These seminars are free of charge, however, registration in event form  is required. Looking forward to seeing you all, LS&E microscopy core team.

 

18/10/2016

Aquifer HIVE technology server platform is now available at the LS&E

The LS&E microscopy unit is happy to announce that HIVE technology is available for IMARIS and Light-sheet users. HIVE is a high speed centralized data repository that removes the need to constantly move or duplicate data sets in processing and analysis workflows The HIVE is a modular platform (computation & storage server) optimized for image processing and data handling. The HIVE designed for the pragmatic, biology-focused scientist, it boosts productivity in the LS&E microscopy unit .Its modular design integrates high speed processing, visualization, remote access, flexibility, data security, scalability and ease of use in one unit.