The LS&E Microscopy core facility the invites You to attend special seminars in Advanced Microscopy Application and Methodologies.
The next seminar will be on “Insights into super-resolution – methodologies and applications of super-resolution microscopy “.
Date: Tuesday, March 21st, 10:00 o’clock
Please keep note that the seminar will be 30 minutes earlier than the original plan.
Location: Auditorium , Faculty of Biology
Speaker: Avi Jacob, Ph.D.
Head of Light Microscopy facility, Bar-Ilan University.
Microscopy-based methods for breaking diffraction limited resolution are collectively known as super-resolution (SR). There are several methods which can be divided into two main approaches: deterministic and stochastic. Stochastic methods include all localization techniques such as STORM and deterministic methods include the optical approaches such as STED. STED (STimulated Emission Depletion) is a mature technology, yet can require optimization to reach ~50nm resolution. Recently, companies have begun to offer “hardware-optimization + software based SR”, whereby a measure of SR is achieved by automatically optimizing acquisition and then submitting to deconvolution. Each has their advantages.
Model systems need to be appropriate for SR, such as sub organelle structures, nuclear pores or fine membrane studies. Also, care must be taken to ensure that SR is technically possible taking into account the specific SR method used. For example, with STED, it’s possible the antibody-secondary-antibody tree could be larger than the airy disk. A balance must be struck between the more time consuming, yet higher resolution STED, and the faster “middle of the way” approach that can be universally used.
Light refreshment will be served before the seminar
Looking forward to seeing you all,
LSE Microscopy team