LSM 510 – Upright confocal with meta detector and mp laser

Description

The LSM 510 META laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked to an upright fully motorized Axio Imager Z1 microscope.

 

Features

  •  Laser options:

The system includes 3 visible lasers and an IR-multi photon laser:

– Multiline Argon laser with excitations at: 458, 477, 488, 514nm (30mW)

– DPSS laser with excitation line at: 561 nm (10mW)

– HeNe laser, with excitation line at: 633 nm (5mW)

– TiSapphire Multi-photon laser with excitation line varied from 690-1040 nm (2.4 W at 800 nm)

  •  Objectives:

 

Magnitude X10 X25 X40 X63 X63 X63
NA 0.3 0.8 1.3 1.0 1.4 1.0
Immersion dry Water ± cover
slip / Glycerol ±
cover slip / Oil
(correction ring)
Oil Water-cover
slip
Oil Water-
dipping

 

  • Controller: Two-mirror scanner system offers 360° XY scanning field rotation, quick zooming, different acquisition modes: simultaneous, sequential, bi-directional and more.
  • Detection: 4 individual fluorescent channels: 3 PMT detectors and 1 Meta detector for spectral imaging. A variety of available emission filters offers imaging of wide fluorochromes from the UV to the IR spectral region 1 transmitted light channel allowing superimposing fluorescence images on bright filed, DIC images.
  • 2-photon application: the TiSapphier laser allows 2-photon excitation which permits dipper penetration into tissues and samples.
  • Acquisition and analysis: acquisition is performed using the ZEN software which provides a wide range of image processing functions: 2D/3D, projection, reconstructing, co localization, intensity measurements and more.
    An additional software license for offline analysis is available on the workstation.
    Files generated by the 510 LSM software can be loaded by the ZEN . Using the ZEN lite one can export specific files to any other required form: JPEG, AVI, etc.

 

Applications

Multi channel fluorescence imaging

3-D reconstruction

Quantitative co-localization

Spectral imaging

Time-series

FRET – fluorescence resonance energy transfer

FRAP – fluorescence recovery after photo-bleaching

Mark and Find

 

Releted Documents

Guided Tour For LSM 510 And Meta

Spectral Imaging

Introduction To LSM

LSM 510 Config Guide

Meta Emission Fingerprinting

Multi Photon Microscopy

Multi Pohton Dye Chart

 

Releted Links

Download ZEN Lite

Know How – Laser Scaning System (Zeiss)