Description

The BD FACSCalibur is an analogue analyzer equipped with 2 lasers permitting detection of 4 colors. The main laser is an air-cooled Argon Ion laser exciting at 488 nm (15mW) and the second laser is a Red Diode exciting at 635 nm (9mW).

 

Features

 

FACSCalibur can measure the following parameters (see configuration):

• Forward Scatter (FSC) expresses the size of a cell.
• Side Scatter (SSC) expresses the granularity of a cell.

Fluorescent parameters:

• FL1 – Green emission (filter: 530/30)
• FL2 – Red/Orange emission (filter: 585/42)
• FL3 – Red emission (filter: 670 LP)
• FL4 – Far Red emission (filter: APC – 661/16 nm)

 

Specifications and performance

Fluorescence sensitivity: 1000 molecules of equivalent soluble fluorescein.

Fluorescence resolution: Coefficient of variation (CV) is <3% full peak for propidium iodide-stained (PI) chicken erythrocyte nuclei (CEN) for FL2-A.

Flow rates

Low: 12µl/min.

Med: 35µl/min.

High: 60µl/min.

 

Applications

Analysis of particles of sizes ranging 0.2-150 microns

Cell cycle and DNA analysis

1-4 colors analysis

 

Releted Documents

FACS Calibur Configuration

 

Releted Links

WinMDI Tutorial and Download (by Perdue)